Home : Research : Licensable Technology : Improve Functionality in Real-Time PCR Applications

Molecular beacons are commonly used in Real-Time PCR, when a fluorescent probe is used as a reporter system. Molecular beacons consist of the stem and the loop structure, which form a hairpin structure. The loop structure is a sequence of nucleotides that is complimentary to a particular gene target that the user wishes to detect with PCR. The stem structure is made up of two complimentary sequences that form a double stranded stem that holds the loop in a stable secondary structure. A fluorophore is linked covalently to the end of one arm and a quencher is linked to the other. Hydrogen bonding between nucleotides is important in determining these structures. When the molecular beacon hybridize to a target strand, they undergo a conformational change that enables them to fluoresce brightly.

A simple approach for modifying Molecular Beacons that improves their performance has been developed at the Penn State Animal Diagnostic Laboratory. Molecular Beacon manufacture chemistry remains the same but performance quality is increased in laboratory situations. The changes in design concept are easily implemented at control points at the time an order is placed with the manufacturer. Improved performance quality experienced by the end user may increase the overall demand for Molecular Beacons.

The researchers have developed multiplex assays that they have used for the detection of viral disease agents. The assays have been effected with tissue and fluid samples obtained from cattle of three distinct bovine respiratory diseases. Additional work has been shown the applicability of these modified molecular beacons for the identification of food-borne zoonotic pathogens.

Matthew D. Smith, Sr. Technology Licensing Officer

Your Name (First Last)*

Your Company

Your Email*

Confirm Email*

Your Message:

*required fields